Aptamer Analysis Services

Expert Aptamer Analysis Services: From Screening to Validation with KMD Bioscience

At KMD Bioscience, we specialize in unlocking the power of aptamers—single-stranded DNA or RNA molecules that bind to specific targets with high affinity and specificity. Our comprehensive Aptamer Analysis Services provide end-to-end solutions, guiding your project from initial discovery through rigorous characterization and validation. We empower researchers in therapeutics, diagnostics, and biotechnology with precise, reliable data to accelerate their development pipelines.

Why Choose Aptamers?
Often termed “chemical antibodies,” aptamers offer unique advantages: reversible denaturation, chemical stability, low immunogenicity, and ease of chemical modification. Our services help you leverage these benefits by ensuring you select and characterize the most effective aptamer for your unique application.

Our Core Aptamer Analysis Services

1. SELEX (Systematic Evolution of Ligands by Exponential Enrichment) Optimization & Monitoring
The journey begins with robust selection. We don’t just perform SELEX; we optimize and monitor it for maximum success.

• Custom Library Design: Tailored oligonucleotide libraries based on your target’s nature (proteins, small molecules, cells).

• Process Monitoring: We use qPCR and high-throughput sequencing (HTS) at critical rounds to monitor enrichment, allowing for data-driven decisions to truncate or continue the selection process efficiently.

• Counter-Selection: Integration of counter-targets to eliminate non-specific binders and enhance specificity from the outset.

2. High-Throughput Sequencing (HTS) & Bioinformatics Analysis
Post-SELEX, we employ cutting-edge Next-Generation Sequencing (NGS) to deconvolute your enriched pools.

• Deep Sequencing: Generates millions of sequences, capturing both abundant and rare aptamer candidates.

• Advanced Bioinformatics Pipeline: Our proprietary analysis clusters sequences into families, identifies consensus motifs, and ranks candidates based on frequency, structure, and potential binding energy.

• Candidate Selection Report: We deliver a curated list of top candidate sequences with analytical insights, providing a strong foundation for downstream validation.

3. Binding Affinity & Specificity Characterization
Quantifying interaction strength is crucial. We offer a suite of biophysical techniques:

• Surface Plasmon Resonance (SPR): Label-free, real-time analysis for determining precise kinetic parameters (Ka, Kd, KD) and specificity.

• Bio-Layer Interferometry (BLI): A flexible, solution-based platform for rapid affinity screening and characterization.

• Isothermal Titration Calorimetry (ITC): Provides a full thermodynamic profile (ΔH, ΔS, ΔG) of the aptamer-target interaction.

• ELISA & Flow Cytometry-Based Assays: For characterizing binding to complex targets like membrane proteins on cells.

4. Structural Analysis & Molecular Modeling
Understanding structure-function relationships is key to optimization.

• Secondary Structure Prediction & Analysis: Using tools like Mfold and NUPACK to predict stable folding patterns.

• Chemical Mapping: Experimental determination of binding regions and conformational changes upon target interaction.

• Molecular Docking & Dynamics Simulations: In silico modeling to visualize and predict binding modes and guide rational design of improved variants.

5. Functional Validation & Assay Development
We ensure your aptamers perform in their intended application.

• Inhibition/Binding Assays: Testing functional efficacy in blocking a protein-protein interaction or enzyme activity.

• Diagnostic Assay Development: Incorporating aptamers into prototype biosensors (colorimetric, fluorescent, electrochemical).

• Cell-Based & In Vivo Imaging: Modifying aptamers with fluorophores or radiolabels for targeted imaging studies.

Our Service Workflow

1. Consultation & Design: We collaborate with you to understand your target and objectives.

2. Aptamer Selection (SELEX): Implementing the optimized selection protocol.

3. Sequencing & In Silico Analysis: Identifying lead candidates.

4. Synthesis & Initial Screening: Chemical synthesis of leads and primary binding tests.

5. Comprehensive Characterization: Full biophysical and functional analysis.

6. Final Reporting & Support: Detailed data package, analytical report, and ongoing project support.

Why Partner with KMD Bioscience?

• Integrated Platform: From selection to validation under one roof, ensuring consistency and saving you time.

• Expert Team: Scientists with deep experience in nucleic acid chemistry, biophysics, and assay development.

• State-of-the-Art Technology: Access to premier platforms like SPR, BLI, NGS, and ITC.

• Custom-Focused: Every project is tailored to your specific target and end-goal, whether it’s a therapeutic antagonist, a diagnostic capture agent, or a research tool.

Advance Your Aptamer Project with Confidence.
Let KMD Bioscience be your partner in navigating the complexities of aptamer development. Our detailed analytical services provide the clarity and confidence needed to move your research forward.

Contact us today to discuss your aptamer analysis needs and request a detailed project proposal.