Integrated high-throughput antibody characterization to advance drug discovery research Surface Plasmon Resonance (SPR) is a powerful analytical technology for analyzing and characterizing antibodies.
SPR measures the antibody-antigen binding kinetics and performs epitope binning and mapping. Benefits of SPR antibody characterization using the Carterra LSA: High-throughput: Kinetically screen and rank up to 1,152 mAbs and 384 clones at one time in parallel. Low sample usage: Only <200µl of each interactant is required. Rapid data analysis: Powerful software tools enable large data sets to be rapidly fitted, visualized, and automatically validated against several data quality parameters. Detailed data mining: Interrogate mAb panels via interactive on-rate, off-rate, affinity, and iso-affinity plots. Data integration: Combine binning and kinetics screening data for comprehensive monoclonal antibody mAb evaluation. Let’s Discuss Your Experiment Antibody screening and kinetics services The Carterra LSA enables high-throughput screening of up to 384 antibodies simultaneously. Click on the image below to see the full results from a single capture kinetics assay where a monovalent target (analyte) was titrated over 384 antibodies, each captured onto individual spots of a 384-array. Each panel represents the global kinetic analysis on a single spot; all 384 spots were analyzed in parallel (some spots are excluded) Screening & Kinetics Enlarged view of the data shown above from three spots showing antibodies that bound their target with diverse kinetics. Request a Quote Epitope mapping and binning services For epitope binning, we test antibodies in a pairwise combinatorial manner. Those that compete for the same binding region are grouped into bins. See the example output data below: We utilize a premix strategy for binning experiments to save time and resources. First, the antigen is saturated with a mAb analyte in solution. This preformed antibody/mAb complex is then tested for binding to an immobilized mAb; its binding response is compared with that of antigen alone.
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