We understand that in the fields of precision medicine, diagnostic development, and drug discovery, researchers need targeting and recognition tools that go beyond traditional antibodies, offering greater programmability and stability. KMD Bioscience provides professional, efficient, and customized one-stop aptamer development services based on mature platform technologies. We are committed to helping you obtain “chemical antibodies” with high affinity and specificity for your target through advanced screening technologies, accelerating your research and translation processes.
Aptamers are a class of single-stranded oligonucleotides (DNA or RNA) that can specifically bind to targets. Their three-dimensional structures can recognize a wide range of targets, including proteins, small molecules, cells, and even microorganisms. Hence, they are hailed as “chemical antibodies.” Compared to antibodies, aptamers offer unique advantages such as chemical synthesis, batch-to-batch consistency, ease of modification, and a broad target range.
The core of our service is based on SELEX (Systematic Evolution of Ligands by Exponential Enrichment) technology. Through in vitro screening, we enrich and identify the aptamer sequences with the strongest binding affinity for your target molecule from vast random sequence libraries.
To meet diverse research needs, we have established various SELEX technology platforms, ensuring the most suitable screening strategy for your target. You can quickly understand our core technologies through the table below:
| Technology Platform | Core Technical Features | Typical Targets/Application Scenarios | Core Advantages |
|---|---|---|---|
| Conventional SELEX | The target molecule is immobilized on a solid-phase carrier, and enrichment is performed through multiple rounds of binding-washing-amplification cycles. | Stable large proteins, intact living cells (Cell-SELEX), virus or pathogen detection. | Mature and stable technology, suitable for various immobilizable targets. |
| Cell-SELEX | Uses intact living cells as screening targets to directly select aptamers that recognize targets in their native conformational state on the cell surface. | Specific recognition of tumor cells, discovery of cell surface markers, targeted drug delivery. | Targets maintain native conformation and modifications; selected aptamers are more suitable for in vivo diagnostics and therapy. |
| Capillary Electrophoresis SELEX (CE-SELEX) | Utilizes differences in electrophoretic mobility between aptamer-target complexes and free nucleic acids in capillary electrophoresis for efficient separation. | Rare or low-abundance target proteins, projects requiring rapid acquisition of high-affinity aptamers. | Few screening rounds required (typically 2-4), extremely high efficiency, significantly saving time and sample consumption. |
| Capture-SELEX | The nucleic acid library is immobilized on a carrier, and target binding-induced conformational changes are used to release specific aptamers. | Small molecule targets difficult to immobilize (e.g., antibiotics, toxins, hormones, metal ions). | Effectively addresses the challenge of immobilizing small molecule targets; ideal for small molecule detection and biosensor development. |
| Negative & Counter-SELEX | Pre- or co-incubates with non-target matrices or structural analogs during screening to remove non-specifically bound sequences. | All projects requiring extremely high specificity, such as distinguishing structurally highly similar protein homologs or isoforms. | Can significantly enhance the specificity of final aptamers, reduce false positives, and potentially improve affinity by over 10-fold. |
We provide complete services from project design to final validation, ensuring professionalism and efficiency.
Project Consultation & Design: Our experts will communicate thoroughly with you to jointly determine the optimal screening strategy and technical pathway based on your target’s properties (size, availability, purity), application direction (diagnostics, therapy, purification), and budget.
Library Preparation & Screening: According to the plan, we will use a starting random library or prepare a customized one, and perform multiple rounds of screening and enrichment using the selected SELEX platform.
Cloning, Sequencing & Analysis: The final enriched nucleic acid pool is cloned, subjected to high-throughput sequencing, and potential candidate aptamer sequences are identified through bioinformatics analysis.
Synthesis, Validation & Optimization: Candidate aptamers are chemically synthesized and their affinity (Kd value) and specificity are validated using various biophysical techniques such as SPR, BLI, and Isothermal Titration Calorimetry (ITC). Upon request, we also offer sequence optimization services including site-directed mutagenesis, truncation, and chemical modifications (e.g., PEGylation, fluorescent labeling, biotinylation) to enhance stability, half-life, or functionality.
Extensive Experience & Professional Platforms: We are not only experts in aptamer screening but also have deep expertise in phage display antibody/peptide screening, protein expression & engineering, and antibody preparation. This cross-platform experience allows us to understand molecular recognition more deeply and provide you with more comprehensive solutions.
Customized Services: We understand the uniqueness of each research project and reject a “one-size-fits-all” approach. We are committed to tailoring the screening strategy for each project and offering flexible, transparent collaboration models.
Reliable Quality & Delivery: We have standardized operational procedures and strict quality control systems to ensure the authenticity and reliability of the delivered aptamer data. We provide detailed project reports including complete protocols, data charts, and conclusions.
If you are seeking novel targeting molecular tools, please feel free to contact us to discuss your research ideas.
This service introduction is based on publicly available information about KMD Bioscience’s technology service platform and a comprehensive understanding of cutting-edge SELEX technologies. The specific service process, timeline, and quotation require final confirmation based on your project requirements.
If you can provide more information about your target molecule (e.g., type, size, availability) and intended application, I can offer more targeted screening strategy recommendations for you.
Aptamer Affinity Optimization
Aptamer Library Construction
Customized Aptamer Selection
High-throughput Aptamer Screening
High-Throughput Sequencing SELEX Aptamer Screening Service
Conventional SELEX Aptamer Screening Service
Negative SELEX Aptamer Screening Service
Toggle-SELEX Aptamer Screening Service
Capture-SELEX Aptamer Screening Service
Surface Plasmon Resonance SELEX Aptamer Screening Service
Capillary Electrophoresis SELEX Aptamer Screening Service
Magnetic Bead-based SELEX Aptamer Screening Service
Toggle-SELEX Aptamer Screening Service
Negative Aptamer Selection- A Practical Guide to Improving Aptamer Specificity in SELEX
selexkmdbio-Cell Nucleic Acid Aptamer Screening Service
Aptamer Screening- Current Methods and Future Trend towards Non-SELEX Approach
Aptamer Screening Service-Subtractive SELEX
Aptamer Screening Service-Counter SELEX
Aptamer Screening Service-HT-SELEX
Aptamer Screening Service-NGS-SELEX
Aptamer Screening Service-Multi-Round SELEX Screening
Whole Cell-SELEX Aptamer Screening Service
Membrane Protein Aptamer Screening Service
Aptamer Screening Service for Drug Discovery
Aptamer Live Cell SELEX Service
Classical SELEX Service for Aptamer
Aptamer Selection and Identification
Aptamer Screening Process and Applications Overview