Aptamer Screening Services for Multiple Targets

Aptamer Screening Services for Multiple Targets

At KMD Bioscience, we understand that the future of diagnostics, therapeutics, and targeted research lies in high-affinity, specific molecular recognition. While antibodies have long been the standard, aptamers—often termed “chemical antibodies”—offer a superior, versatile alternative. Our comprehensive Aptamer Screening Services are designed to discover and develop these powerful single-stranded DNA or RNA molecules against a diverse range of your targets.

Why Choose Aptamers?
Aptamers bind to their targets, from small molecules and proteins to whole cells and viruses, with exceptional specificity and affinity. They offer distinct advantages:

• High Specificity & Affinity: Selected through an iterative process to precisely recognize unique epitopes.

• Chemical Stability: Unlike proteins, aptamers are thermally stable and can be easily regenerated.

• Easy Modification: Simple chemical synthesis allows for easy labeling and conjugation without loss of activity.

• Low Immunogenicity: Ideal for in vivo therapeutic and diagnostic applications.

Our Multi-Target Screening Platform: SELEX Evolved

We employ and continuously optimize state-of-the-art SELEX (Systematic Evolution of Ligands by EXponential enrichment) technologies to cater to the unique nature of each target. Our platform is not one-size-fits-all; it is a flexible, sophisticated system capable of handling multiple target types:

1. Protein Targets: For biomarker detection, assay development, and therapeutic blocking. We screen against purified recombinant proteins, complex protein mixtures, or specific protein domains.

2. Small Molecule Targets: Ideal for environmental monitoring, food safety, and drug detection. We develop aptamers for haptens, toxins, hormones, and metabolites.

3. Cellular Targets (Cell-SELEX): For identifying aptamers that distinguish between cell states (e.g., cancerous vs. healthy), specific cell surface markers, or pathogen-infected cells—all without prior knowledge of the membrane proteome.

4. Complex Targets: Including viruses, bacteria, exosomes, and other nanostructures, enabling recognition of complex surface architectures.

Our End-to-End Service Workflow

1. Consultation & Design: Our experts work with you to define the target, desired aptamer properties (Kd, specificity, modification), and the final application (biosensor, therapeutic, imaging agent).

2. Library & Strategy Selection: We utilize vast randomized oligonucleotide libraries and choose the optimal SELEX strategy: Magnetic Bead-based SELEX, Capillary Electrophoresis-SELEX (CE-SELEX) for superior stringency, or advanced in silico design and NGS-aided maturation.

3. Iterative Selection & Amplification: The target is incubated with the library, bound sequences are partitioned, and PCR-amplified over multiple rounds under increasingly stringent conditions to enrich high-binders.

4. High-Throughput Sequencing & Bioinformatics: Next-Generation Sequencing (NGS) of enriched pools, coupled with our proprietary bioinformatics analysis, identifies candidate aptamer families and consensus structures.

5. Characterization & Validation: Lead aptamers are synthesized and rigorously validated for:

   • Binding Affinity (Kd): Measured via ELISA, SPR, or BLI.

   • Specificity & Cross-Reactivity: Tested against related targets.

   • Functional Activity: Validated in your intended application format.

Your Partner from Discovery to Application

KMD Bioscience is more than a service provider; we are your collaborative partner in aptamer discovery. Our scientists ensure seamless project management, transparent communication, and delivery of fully characterized, application-ready aptamer sequences with comprehensive data reports.

Unlock the potential of aptamers for your multi-target project. Whether you are developing a multiplex diagnostic panel, a suite of research tools, or exploring novel therapeutic agents, our tailored screening services provide the precision and power you need.

Contact KMD Bioscience today to discuss your targets and initiate your custom aptamer discovery project.

Visit: https://selexkmdbio.com/