Aptamer Screening Services for Protein Targeting

Precision Targeting, Unlocking New Dimensions in Protein Research

In the fields of life science research and biopharmaceutical development, there is a growing demand for molecular tools with high affinity and specificity that target specific proteins. Leveraging our advanced SELEX (Systematic Evolution of Ligands by Exponential Enrichment) technology platform, KMD Bioscience provides professional and efficient aptamer screening services for protein targets, empowering your research to break through technical barriers.

What are Aptamers?

Aptamers are single-stranded DNA or RNA oligonucleotides obtained through in vitro screening techniques. They can bind to specific target molecules—including proteins, small molecules, and cells—with high affinity and specificity. Compared to antibodies, aptamers offer unique advantages such as small molecular weight, high stability, ease of chemical modification, no immunogenicity, and minimal batch-to-batch variation, earning them the title of “chemical antibodies.”

Our Technological Advantages

1. Advanced SELEX Technology Platform

• Multiple SELEX Variants: Including magnetic bead SELEX, capillary electrophoresis SELEX, cell-SELEX, and more, selecting the optimal screening strategy based on target characteristics.

• Next-Generation Sequencing (NGS) Support: Combined with high-throughput sequencing technology for in-depth analysis of screening libraries, significantly improving screening efficiency and success rates.

• Microfluidic Chip Technology: Enables ultra-low volume reaction systems, reducing sample consumption and enhancing screening precision.

2. Extensive Experience with Protein Targets

We have successfully screened high-performance aptamers for a wide range of protein targets, including but not limited to:

• Cell surface receptors (GPCRs, tyrosine kinase receptors, etc.)

• Cytokines and growth factors

• Enzymes (kinases, proteases, etc.)

• Tumor markers

• Viral proteins

• Membrane proteins (with specialized processing)

3. Rigorous Quality Control System

• Real-time monitoring of enrichment progress during screening

• Affinity validation via Surface Plasmon Resonance (SPR) or Bio-Layer Interferometry (BLI)

• Cross-reactivity testing to ensure specificity

• Functional activity validation (e.g., enzyme inhibition, receptor blockade)

Service Process

Phase 1: Project Consultation and Design

• Analysis of target protein characteristics and screening strategy development

• Design of aptamer modification plans based on research applications (diagnostics, therapeutics, imaging, etc.)

• Determination of screening library type (DNA/RNA/chemically modified libraries)

Phase 2: Aptamer Screening

1. Library Design and Synthesis: Standard or customized library construction

2. In Vitro Screening: Multiple rounds of positive and negative screening

3. Cloning and Sequencing: Acquisition and preliminary analysis of candidate sequences

Phase 3: Characterization and Optimization

1. Affinity Determination: Measurement of Kd values (typically reaching nM or even pM levels)

2. Specificity Verification: Cross-reactivity testing against structural analogs and unrelated proteins

3. Stability Assessment: Serum stability, thermal stability, etc.

4. Functional Validation: Application-specific testing based on client requirements

Phase 4: Delivery and Application Support

• Comprehensive experimental reports and data analysis

• Aptamer sequences and synthesis information

• Bulk synthesis support

• Guidance on subsequent modifications and labeling

Application Areas

Diagnostic Development

• Rapid test strips (lateral flow assays)

• Biosensors

• In vitro diagnostic reagents

Therapeutic Research

• Targeted drug delivery

• Receptor antagonists/agonists

• Checkpoint inhibitors

Basic Research

• Protein localization and imaging

• Protein function regulation

• Signaling pathway studies

Industrial Applications

• Ligands for protein purification in bioprocessing

• Quality control detection tools

Success Stories

We have successfully collaborated with numerous academic institutions, biotech companies, and pharmaceutical enterprises worldwide. Selected examples include:

1. Tumor Marker Detection Aptamer: Screened a high-affinity aptamer against PD-L1 (Kd=2.3 nM) for a biotech company, successfully applied in a liquid biopsy platform.

2. Neurodegenerative Disease Research Tool: Developed an RNA aptamer targeting tau protein for a research institution, aiding in Alzheimer’s disease mechanism studies.

3. Viral Detection Aptamer: DNA aptamer targeting the SARS-CoV-2 spike protein, with a detection limit as low as 0.1 ng/mL.

Why Choose KMD Bioscience?

• Expert Team: Led by Ph.D.-level scientists with over a decade of aptamer screening experience.

• Customized Services: Tailored solutions based on your specific needs and research goals.

• Rapid Delivery: Standard process delivers validated candidate aptamers in just 8–12 weeks.

• Confidentiality Agreements: Strict protection of client intellectual property and data security.

• Global Service: Supports worldwide sample shipping and data transfer.

Start Your Project

Whether you are in the exploratory research phase or at a critical stage of product development, our aptamer screening services will provide reliable technical support. Our team of technical consultants offers free preliminary consultations to develop the optimal screening strategy based on your target characteristics and application needs.

Contact Us:
Visit our website at https://selexkmdbio.com/ for more information.
Or email us directly at aptamer-joyce@corenand.com.