1. What is the cell aptamer screening service, and what are the advantages of cell aptamers? A: Our common aptamers include RNA aptamers and DNA aptamers, which targets have high affinity and specificity and are capable of high binding to the target. These aptamers have multiple functions in disease treatment and scientific research. First, it acts as a cell agonist to activate cell receptors and promote the cell to exert its effects. Secondly, it has the effect of antagonist to block the mutual binding and interaction between various structures. Furthermore, the aptamer can bind to the target so that the drug can be accurately transmitted to the therapeutic target. During the cell-SELEX screening, intact live cells were screened as a target, while the associated cell lines were used as negative controls for negative screening to exclude non-specific binding. The Cell-SELEX technology targets multiple cellular targets to generate nucleic acid aptamers, thus providing an advantage in identifying cells as molecules. In disease diagnosis, we can use aptamers for labeling, or use aptamers as a fixative to depurify the cells. These properties of aptamers provide new ideas for novel drug development. One of the highlights of the Cell-SELEX technology is the ability to retain the original conformation of the cellular receptor, which is different from screening…
Steps Service Content Timeline Step 1: Screening of nucleic acid aptamers (1) Cells were provided by the customer. (2) Adaptation library screening and enrichment: PCR amplification enrichment+transcription+gel running recovery, usually 6-10 rounds. (3) Screening products for NGS sequencing. (4) Delivery: 5-15 adapter sequences, experimental report, raw data (including NGS sequencing raw data and gel electrophoresis) 10-15 weeks Step2：Aptamer assay and FACS(optional) (1) Synthesize aptamers based on sequences. (2) Affinity determination of adapter and FACS. (3) Delivery: Experimental report, raw data 4-5 weeks
The specific screening process and sample requirements will vary according to the target characteristics and experimental requirements. The process of cell targeting aptamers screening is mainly based on SELEX technology and optimized using the aptamer cell selex method to meet the screening needs at the cellular level. A aptamer library containing a large number of random nucleic acid sequences (DNA or RNA, typically between 20 and 60 nucleotides in length) is constructed. Specific molecules on target cells or cell surfaces are used as targets (cell culture, purification, or labeling). The target cells are mixed with nucleic acid sequences from the aptamer library, and after incubation, the nucleic acid sequences bind to molecules on the surface of the target cells. The nucleic acid sequence that binds to the target is separated through filtration, centrifugation, magnetic bead separation, and other methods. The combined nucleic acid sequence is eluted and collected for the next round of screening. The secondary library is generated by amplifying the eluted nucleic acid sequence using PCR technology. The steps of binding, separation, elution, and amplification are repeated to gradually enrich high affinity aptamers. Through sequencing and sequence analysis, candidate aptamers were identified from the final enriched library. Simply put, SELEX…
Nucleic Acid Aptamer Synthesis Service Advantages ✔ The library has a capacity of 10 ^ 13-10 ^ 14, sufficient to screen for nucleic acid aptamers targeting customer targets. ✔ SELEX technology platform mature: the affinity of nucleic acid aptamers obtained through screening can reach the nM-pM level. ✔ Traceability of experimental records: QC quality control standards, Chinese and English experimental reports, original experimental records. ✔ One on one personalized solution customization (including screening and subsequent verification plans, etc.) to meet the research project needs of various clients. ✔6-10 rounds of pressure screening can obtain high affinity and high specificity nucleic acid aptamers.
