Customized Aptamer Selection

Customized Aptamer Selection refers to a tailored process of identifying and developing aptamers—short, single-stranded DNA or RNA molecules—that specifically bind to a target molecule (proteins, small molecules, cells, or pathogens) according to a client’s specific requirements. Unlike standard aptamer screening, it focuses on individualized targets, binding conditions, and functional needs.

Key Features:

1. Target Specificity: Aptamers are selected for high affinity and specificity to a particular target.

2. Flexible Design: Can be designed for proteins, peptides, small molecules, ions, or whole cells.

3. Binding Conditions Customization: pH, temperature, ionic strength, or buffer system can be tailored.

4. Functional Application: Aptamers can be developed for diagnostics, therapeutics, biosensors, or research.

5. High-Throughput & Efficiency: Advanced techniques allow rapid screening for optimal aptamers.

Typical Workflow:

1. Target Analysis: Understanding target structure and function.

2. Library Preparation: Generate a diverse pool of oligonucleotides.

3. SELEX (Systematic Evolution of Ligands by EXponential enrichment): Iterative selection process to enrich high-affinity aptamers.

4. Binding Affinity Testing: Determine Kd (dissociation constant) and specificity.

5. Sequence Optimization & Modification: Chemical modifications for stability or functionalization.

6. Delivery of Customized Aptamer: Ready for research, diagnostics, or therapeutic use.

Common Applications:

• Diagnostics: Biosensors for disease markers.

• Therapeutics: Targeted drug delivery.

• Research Tools: Protein purification or molecular imaging.

• Environmental Monitoring: Detection of pollutants or toxins.