Nucleic Acid Aptamer Synthesis Service Advantages ✔ The library has a capacity of 10 ^ 13-10 ^ 14, sufficient to screen for nucleic acid aptamers targeting customer targets. ✔ SELEX technology platform mature: the affinity of nucleic acid aptamers obtained through screening can reach the nM-pM level. ✔ Traceability of experimental records: QC quality control standards, Chinese and English experimental reports, original experimental records. ✔ One on one personalized solution customization (including screening and subsequent verification plans, etc.) to meet the research project needs of various clients. ✔6-10 rounds of pressure screening can obtain high affinity and high specificity nucleic acid aptamers.
Nucleic acid aptamers exhibit exceptional specificity in recognizing and binding to their target molecules, allowing them to pinpoint targets amidst a myriad of molecules, effectively sidestepping the disruptions posed by nonspecific binding. Their binding capacity with target molecules is robust, sometimes even surpassing that of antibodies. This superior affinity renders nucleic acid aptamers exceptionally sensitive and precise in applications like detection and diagnosis. SELEX technology offers a highly efficient and targeted approach for the screening of nucleic acid aptamers, thereby advancing their utilization and progress across diverse domains. KMD Bioscience employs this SELEX technology to identify aptamers with strong affinity for specific, targeted substances from a randomly generated library of single-stranded nucleic acid aptamers. KMD Bioscience uses advanced SELEX screening technology to accurately extract oligonucleotides with high affinity for targets from a large random library. After multiple rounds of screening cycles, SELEX fragments were sequenced based on their enrichment levels to obtain adapter sequences (RNA aptamers, DNA aptamers). Among them, the oligonucleotide library has been uniquely designed with fixed sequences at both ends and cleverly inserted with random sequences (RNA aptamers, DNA aptamers) in the middle. In the fixed sequence section, primer binding sites that are conducive to PCR amplification…
KMD Bioscience, with years of research experience in the field of aptamer development, has successfully built a comprehensive aptamer library system and accumulated profound project practical knowledge in the process. KMD Bioscience can provide customers with excellent aptamer library construction services and support subsequent research processes such as aptamer screening, nucleic acid aptamer sequence synthesis, functional validation, and aptamer development for specific molecules. KMD Bioscience has achieved sequence optimization, upgrading, and significant functional enhancement through professional nucleic acid adapter sequence synthesis services. With the help of an advanced adapter library construction platform, KMD Bioscience ensures that customers can obtain efficient and accurate nucleic acid adapter results. In addition, KMD Bioscience also has the ability to provide comprehensive aptamer library design services, covering the entire process from gene analysis synthesis, in vitro screening of aptamers, aptamer synthesis to affinity determination, striving to meet the diverse project needs of various customers. The nucleic acid aptamer library constructed by KMD Bioscience covers a wide range of sequences and has a broad coverage. The core elements such as sequence length, GC ratio, and secondary structure in the library have been optimized to enrich the diversity of aptamers while strictly monitoring their quality. Based on years…
KMD Bioscience can provide a range of services for aptamer development, SELEX screening and optimization of aptamers, which are mainly based on aptamer SELEX screening technology. The target molecules of aptamer range widely, including small molecules such as metal ions, amino acids and nucleotides, biological macromolecules such as growth factors, proteins and peptides, and even viruses, bacteria, living cells, tissue sections and living organisms. KMD Bioscience can provide suitable aptamer SELEX screening services for a variety of targets, including peptides, and continue to advance in aptamer development. Peptides are polymers formed by covalent linkage of two or more amino acids through peptide bonds. According to the number of amino acid residues, peptides can be divided into oligopeptides (generally composed of 2 to 10 amino acids) and peptides (composed of more than 10 amino acids). Peptides have a variety of functions in organisms, such as hormones, neurotransmitters, growth factors, etc. They play an important role in regulating the physiological activities of organisms and participating in metabolic processes. Nucleic acid aptamers obtained by screening peptides have a variety of uses. For example, based on its recognition function, it can be used as an affinity reagent to establish analytical detection methods or carry out biological imaging studies. Based…
Advantages of Cellular Aptamer Screening Service FAQ-Cell Nucleic Aptamer What is the cell aptamer screening service, and what are the advantages of cell aptamers? A: Our common aptamers include RNA aptamers and DNA aptamers, which targets have high affinity and specificity and are capable of high binding to the target. These aptamers have multiple functions in disease treatment and scientific research. First, it acts as a cell agonist to activate cell receptors and promote the cell to exert its effects. Secondly, it has the effect of antagonist to block the mutual binding and interaction between various structures. Furthermore, the aptamer can bind to the target so that the drug can be accurately transmitted to the therapeutic target. During the cell-SELEX screening, intact live cells were screened as a target, while the associated cell lines were used as negative controls for negative screening to exclude non-specific binding. The Cell-SELEX technology targets multiple cellular targets to generate nucleic acid aptamers, thus providing an advantage in identifying cells as molecules. In disease diagnosis, we can use aptamers for labeling, or use aptamers as a fixative to depurify the cells. These properties of aptamers provide new ideas for novel drug development. One of the highlights of the Cell-SELEX technology is the ability to retain the original conformation…
The specific screening process and sample requirements will vary according to the target characteristics and experimental requirements. The process of cell targeting aptamers screening is mainly based on SELEX technology and optimized using the aptamer cell selex method to meet the screening needs at the cellular level. A aptamer library containing a large number of random nucleic acid sequences (DNA or RNA, typically between 20 and 60 nucleotides in length) is constructed. Specific molecules on target cells or cell surfaces are used as targets (cell culture, purification, or labeling). The target cells are mixed with nucleic acid sequences from the aptamer library, and after incubation, the nucleic acid sequences bind to molecules on the surface of the target cells. The nucleic acid sequence that binds to the target is separated through filtration, centrifugation, magnetic bead separation, and other methods. The combined nucleic acid sequence is eluted and collected for the next round of screening. The secondary library is generated by amplifying the eluted nucleic acid sequence using PCR technology. The steps of binding, separation, elution, and amplification are repeated to gradually enrich high affinity aptamers. Through sequencing and sequence analysis, candidate aptamers were identified from the final enriched library. Simply put, SELEX technology…
As a functional nucleic acid, aptamer has many advantages. Based on its high specificity, it can accurately find the target molecule, thus avoiding the interference caused by nonspecific binding. Through in vitro selection technology (SELEX) screening, aptamers can be synthesized rapidly and in large quantities without complex biological preparation process. Aptamers have good chemical stability, can keep their structure and function unaffected under a variety of environmental conditions, and have higher thermal stability and lower immunogenicity. The molecular weight of aptamers is relatively small, making it easier for them to penetrate cell and tissue barriers and reach the site of action quickly. The core of aptamer screening is SELEX technology, which can accurately screen nucleic acid molecules that can achieve highly specific binding with specific targets from a large number of nucleic acid libraries with diverse sequences after multiple rounds of selection and amplification cycles. DNA or RNA aptamers obtained by in vitro screening technology have a unique three-dimensional structure, which can accurately identify and tightly bind to the corresponding target molecules. Compared with traditional antibodies, aptamers exhibit a variety of advantages, such as thermal stability, chemical synthesis and modification ability, and low immunogenicity. These characteristics together determine the great…
Cell Nucleic Acid Aptamer Screening Service KMD Bioscience has many years of research experience in the field of drug antibodies, and has accumulated profound experience in antibody development, aptamer screening, aptamer assay, affinity maturation, etc. KMD Bioscience can provide aptamer screening services for a variety of sample types, such as proteins, peptides, amino acids, small molecular substances, cells and bacteria, metal ions, etc. KMD Bioscience can provide customized in vitro aptamer screening services to ensure efficient, accurate and rapid screening of aptamer sequences required by customers. In addition, KMD Bioscience can provide customers with upstream and downstream services covering aptamer screening, from gene analysis and synthesis, aptamer in vitro screening, aptamer synthesis, aptamer assay, to affinity determination. KMD Bioscience can provide strong support for subsequent functional verification of aptamers (including but not limited to affinity verification, competitive ELISA verification, in vitro targeted cell functional verification (such as in vitro recognition and inhibition function verification of nucleic acid aptamers, in vitro flow cytometry blocking function verification, etc.), and in vivo functional verification (such as in vivo targeted inhibition function verification of aptamers, signal pathway blocking function verification, etc.), laying a solid foundation for subsequent work of customers, such as downstream research and development of targeted specific molecular drugs. The nucleic acid aptamer screening…
Current Status and Future Directions of Aptamer Development Current Status Aptamers (often called "chemical antibodies") are single-stranded DNA or RNA oligonucleotides that bind to specific targets with high affinity and specificity. They are selected via SELEX (Systematic Evolution of Ligands by Exponential Enrichment). Key Achievements: Therapeutic Applications: The first FDA-approved aptamer drug, Pegaptanib (Macugen), treats age-related macular degeneration by targeting VEGF. Diagnostics: Aptamers are used in biosensors (aptasensors) for detecting proteins, small molecules, and pathogens (e.g., COVID-19 detection platforms). Target Range: Successfully generated against diverse targets: ions, small molecules, proteins, cells, and even whole organisms. Technical Advances: Development of Cell-SELEX, in vivo SELEX, and high-throughput SELEX has expanded capabilities. Advantages Over Antibodies: Chemical synthesis: No batch-to-batch variation. Modifiability: Can be chemically modified for stability (e.g., nuclease resistance) and functionality. Wider target range: Can bind toxins or non-immunogenic targets. Current Challenges: Stability issues (especially RNA aptamers degrade in biological fluids). Limited commercialization beyond a few successes. SELEX process can be time-consuming and biased. Delivery challenges for therapeutic applications. Future Directions 1. Enhanced Selection Technologies Machine learning-guided SELEX: Using AI to predict binding motifs and reduce selection cycles. Microfluidic SELEX: Miniaturized, automated platforms for faster, more efficient selection. In vivo SELEX: Direct selection in living organisms for better therapeutic relevance. 2. Chemical Modifications &…
Of course. Aptamers, often called "chemical antibodies," are single-stranded DNA or RNA oligonucleotides that bind to specific targets with high affinity and specificity. Their unique properties—including in vitro selection (SELEX), chemical stability, low immunogenicity, and ease of modification—make them versatile tools across numerous fields. Here are the major application fields of aptamers, categorized for clarity: 1. Therapeutics This is one of the most active areas, with one approved drug (Macugen® for macular degeneration) and many in clinical trials. Antagonistic Therapeutics: Blocking the function of disease-related proteins (e.g., receptors, cytokines). Targeted Drug Delivery: Aptamers are conjugated to drugs, toxins, or nanoparticles to selectively deliver them to diseased cells (e.g., cancer cells), minimizing systemic side effects. Agonistic Therapeutics: Activating specific receptors to trigger therapeutic pathways. Antiviral/Antibacterial Agents: Binding to viral surface proteins or bacterial toxins to neutralize them. 2. Diagnostics and Biosensing Aptamers are core components of novel, rapid, and sensitive diagnostic platforms. Point-of-Care (POC) Tests: Used in lateral flow assays (like advanced pregnancy tests) for detecting pathogens (e.g., SARS-CoV-2), toxins, or biomarkers. Electrochemical and Optical Biosensors: Aptamers immobilized on electrodes or chips change signals upon target binding, enabling detection of targets from small molecules to whole cells. Medical Imaging: Radiolabeled or fluorescently labeled aptamers (optamers) act as contrast agents…
