SPOT Synthesis (often written as SPOT peptide synthesis or SPOT synthesis technique) is a positionally addressable, parallel solid-phase peptide synthesis method where many peptides are built simultaneously as discrete “spots” on a derivatized cellulose membrane. Instead of synthesizing one peptide at a time on resin beads, SPOT Synthesis dispenses activated amino acid solutions onto predefined membrane coordinates, enabling rapid generation of peptide libraries and arrays for downstream screening.  ⸻ What Makes SPOT Synthesis Unique? 1) Parallel synthesis on a planar cellulose support In SPOT Synthesis, the membrane acts as a flat solid support. Each printed droplet is absorbed into the porous cellulose and behaves like a tiny reaction “micro-compartment,” allowing hundreds to thousands of peptides to be synthesized in parallel on one sheet.  2) Addressable peptide libraries (arrays you can map by position) Every spot corresponds to a known sequence (or sequence mixture), which makes SPOT arrays especially useful when you need systematic coverage—such as scanning a protein sequence with overlapping peptides or exploring sequence–activity relationships.  3) Scale and throughput The method is widely described as supporting very high spot counts (from hundreds up to many thousands, depending on format and spot size). This density makes it…
