aptamer screening service-Aptamer Development Service Workflow

Custom Aptamer Discovery Service: Our Tailored Development Process

Curious about how we identify precision-targeting aptamers? Our step-by-step workflow is designed to isolate high-affinity molecular binders for your specific target.

Our Proven Development Pathway:

1. Design & Synthesis of Custom ssDNA Library
We construct a diverse single-stranded DNA library with randomized sequences, tailored to your target’s properties for optimal screening potential.

2. Target-Specific Binding Selection
Your target molecule (protein, small molecule, or cell) is incubated with our curated DNA library under optimized conditions to capture specific binders.

3. Rigorous SELEX Enrichment Cycles
Through multiple iterative rounds of:

• Stringent Washing – Removal of non-specific binders

• Selective Elution – Recovery of target-bound sequences

• Controlled PCR Amplification – Enrichment of high-affinity candidates

4. Next-Generation Sequencing & Bioinformatics Analysis
Deep sequencing of enriched pools followed by advanced computational analysis to identify conserved binding motifs and candidate families.

5. Comprehensive Affinity Validation
Each candidate undergoes thorough characterization including:

• Kd Determination – Precise binding affinity measurement

• Specificity Profiling – Cross-reactivity assessment

• Functional Validation – Performance evaluation in your intended application format

Deliverables:

• Fully characterized aptamer sequences with binding parameters

• Specificity validation data

• Optimization for your required buffer conditions

• Technical support for implementation

Our Advantage: Proprietary screening methodologies, stringent counter-selection protocols, and integrated computational design ensure we deliver aptamers with superior specificity and nanomolar-range affinity for your unique applications.