Metal Ion Nucleic Acid Aptamer Screening Service Workflow

Date：2025-12-31

Metal Ion Nucleic Acid Aptamer Screening Service Workflow

Step Service Content Timeline

Step 1: Screening of nucleic acid aptamers (1) Customer provides screening targets.

(2) The adapter library is fixed on an affinity chromatography column and incubated with metal ions injected into the column.

(3) Adaptation library screening and enrichment: PCR amplification enrichment+transcription+gel running recovery, usually 6-10 rounds.

(4) Screening products for NGS sequencing.

(5) Delivery: 5-15 adapter sequences, experimental report, raw data (including NGS sequencing raw data and gel electrophoresis)
10-15 weeks

Step2：Synthesis of aptamers and determination of affinity (optional) (1) Synthesize aptamers based on sequences.

(2) Affinity determination of adapter and target protein, KD determination by BLI or SPR.

(3) Delivery: Experimental report, raw data